anti-Eg 5 antibody

Key features and details

  • 产品描述: Mouse Monoclonal antibody recognizes Eg 5
  • 反应物种: Hu
  • 应用: ICC/IF, IP, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 同位型: IgG1
  • 靶点名称: Eg 5
  • 抗原物种: Human
  • 抗原: Purified recombinant human Eg5 protein fragments expressed in E.coli.
  • Brand:
CAT.NO. : ARG53992
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Product Details
概述
产品描述Mouse Monoclonal antibody recognizes Eg 5
反应物种Hu
应用ICC/IF, IP, WB
宿主Mouse
克隆Monoclonal
同位型IgG1
靶点名称Eg 5
抗原物种Human
抗原Purified recombinant human Eg5 protein fragments expressed in E.coli.
偶联标记Un-conjugated
別名TRIP-5; Kinesin-related motor protein Eg5; KNSL1; TR-interacting protein 5; HKSP; Kinesin-like spindle protein HKSP; EG5; MCLMR; Kinesin-like protein 1; Kinesin-like protein KIF11; TRIP5; Thyroid receptor-interacting protein 5
应用说明
应用建议
应用推荐稀释比
ICC/IF1:200
IPAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量130 kDa
属性
形式Liquid
纯化Affinity purified
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度Lot dependent
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3832 Human KIF11

Swiss-port # P52732 Human Kinesin-like protein KIF11

基因名称KIF11
全名kinesin family member 11
背景介绍Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
生物功能Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. [UniProt]
细胞定位Cytoplasm
研究领域Gene Regulation antibody; Signaling Transduction antibody
预测分子量119 kDa
翻译后修饰Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
检测图片 (3)
  • ARG53992 anti-Eg 5 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG53992 anti-Eg 5 antibody at 1:200 dilution.

  • ARG53992 anti-Eg 5 antibody WB image

    Western blot: MCF7 cell lysate stained with ARG53992 anti-Eg 5 antibody at 1:1000 dilution.

  • ARG53992 anti-Eg 5 antibody IP image

    Immunoprecipitation: Jurkat cell lysates were immunoprecipitated and stained with ARG53992 anti-Eg 5 antibody.

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