Anti-EGFRvIII Rabbit Monoclonal Antibody [Clone RM419], 31-1305-00 31-1305-02
![Anti-EGFRvIII Rabbit Monoclonal Antibody [Clone RM419], 31-1305-00 31-1305-02](/upload/image/products/RM419_IHC_GBM-pos-copy-e1591400636574.png)
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CAT.NO. : 31-1305-02
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Product Details
Product Details
| Product Name | Anti-EGFR Variant III Rabbit Monoclonal Antibody, Clone RM419 |
| Product Description | Rabbit monoclonal to Epidermal Growth Factor Receptor Variant III |
| Catalog No. | 31-1305-00 31-1305-02 |
| Clone Name | RM419 |
| Product Data Sheet | Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419 Biotinylated Anti-EGFR Variant III Rabbit Monoclonal Antibody Data Sheet RM419 |
| Specificity | RM419 reacts to human Epidermal Growth Factor Receptor Variant III.No cross reactivity with wild type EGF receptor. |
| Immunogen | A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII) |
| Application | IHC, WB, ELISA, FC |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µL; Biotinylated 50 µL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | IHC: 1:100 – 1:200 dilution; FC: 1:10 – 1:100 dilution; WB: 1:1000 - 1:2000 dilution; ELISA: 1:100 – 1:1000 dilution. |
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Product Images
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using anti-EGFRvIII antibody (Clone RM419) at 1:100 dilution. |
 | Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates, using anti-EGFRvIII antibody (Clone RM419) and anti-EGFRct (Clone RM294) at 1:1000 dilution. |
 | Flow cytometry analysis of DKMG and 293 cells, using anti-EGFRvIII antibody (Clone RM419) at 1:50 dilution. |
 | ELISA analysis showing RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (Clone RM419) or anti-EGFR (Clone RM229) antibodies were used. |
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