anti-eIF4E antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG55572
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody recognizes eIF4E |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 163CT48.1.9 |
| 同位型 | IgG1, kappa |
| 靶点名称 | eIF4E |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human eIF4E. |
| 偶联标记 | Un-conjugated |
| 別名 | EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; CBP; eIF-4E; eIF4E; AUTS19; EIF4F |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Jurkat |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Crude Ascites and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P06730 Human Eukaryotic translation initiation factor 4E Swiss-port # P63073 Mouse Eukaryotic translation initiation factor 4E |
|---|---|
| 基因名称 | EIF4E |
| 全名 | eukaryotic translation initiation factor 4E |
| 背景介绍 | The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| 生物功能 | Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt] |
| 细胞定位 | Cytoplasm, P-body. Cytoplasm |
| 研究领域 | Gene Regulation antibody |
| 预测分子量 | 25 kDa |
| 翻译后修饰 | Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex. |
检测图片 (2)
ARG55572 anti-eIF4E antibody WB image
Western blot: 15 µg of Jurkat cell lysate stained with ARG55572 anti-eIF4E antibody.
ARG55572 anti-eIF4E antibody FACS image
Flow Cytometry: Jurkat cells stained with ARG55572 anti-eIF4E antibody (right histogram) or without primary antibody as control (left histogram), followed by incubation with FITC labelled secondary antibody.
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