anti-eIF5 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes eIF5
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: eIF5
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 1-235 of Human eIF5 (NP_892116.2).
  • Brand:
CAT.NO. : ARG58659
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes eIF5
反应物种Hu, Ms, Rat
应用ICC/IF, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称eIF5
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 1-235 of Human eIF5 (NP_892116.2).
偶联标记Un-conjugated
別名Eukaryotic translation initiation factor 5; eIF-5; EIF-5; EIF-5A
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照HeLa
实际分子量55 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 1983 Human EIF5

GeneID: 217869 Mouse EIF5

GeneID: 56783 Rat EIF5

基因名称EIF5
全名eukaryotic translation initiation factor 5
背景介绍Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]
生物功能Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). [UniProt]
预测分子量49 kDa
检测图片 (2)
  • ARG58659 anti-eIF5 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG58659 anti-eIF5 antibody.

  • ARG58659 anti-eIF5 antibody WB image

    Western blot: 25 µg of HeLa cell lysate stained with ARG58659 anti-eIF5 antibody at 1:1000 dilution.

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