anti-EMR1 F4 / 80 antibody [Cl:A3-1]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Rat Monoclonal antibody [Cl:A3-1] recognizes EMR1 F4 / 80 |
|---|---|
| 反应物种 | Ms, Rat |
| 应用 | EM, FACS, ICC/IF, IHC-Fr, IHC-P, IHC-R, IP, RIA, WB |
| 特异性 | EMR1 F4/80 antibody, clone Cl:A3-1, is the best macrophage and microglial marker. It recognises the murine F4/80 antigen, a 160 kD cell surface glycoprotein, member of the EGF-TM7 family. |
| 宿主 | Rat |
| 克隆 | Monoclonal |
| 克隆号 | Cl:A3-1 |
| 同位型 | IgG2b |
| 靶点名称 | EMR1 F4 / 80 |
| 抗原物种 | Mouse |
| 抗原 | Thioglycollate stimulated peritoneal macrophages from C57BL/6 mice. |
| 偶联标记 | Un-conjugated |
| 別名 | EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; EMR1; Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; TM7LN3 |
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Proteinase K is recommended for tissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixed for more than 24 hours. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 基因名称 | Adgre1 |
|---|---|
| 全名 | adhesion G protein-coupled receptor E1 |
| 背景介绍 | EMR1 F4/80 has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | EMR1 F4/80: Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30326 Inflammatory Cell Antibody Panel (for mouse) Related products: EMR1 F4/80 antibodies; EMR1 F4/80 Duos / Panels; Anti-Rat IgG secondary antibodies; Related news: Exploring Antiviral Immune Response |
| 研究领域 | Mouse Inflammatory Cell Marker antibody; Macrophage Marker antibody |
| 预测分子量 | 98 kDa |
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] ICC/IF image
Immunofluorescence: Rat tumor macrophages stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1].
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image
Immunohistochemistry: Mouse spleen cryosection stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-Fr image
Immunohistochemistry: Frozen section of Mouse liver tissue stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution.
ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] IHC-P image
Immunohistochemistry: A. Mouse spleen stained with ARG22476 anti-EMR1 F4/80 antibody [Cl:A3-1] at 1:100 dilution. B. Primary antibodies preincubated with 2 molar excess of Human anti Idiotypic.
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