anti-ENO3 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes ENO3
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Bov, Pig
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: ENO3
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG55574
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes ENO3
反应物种Hu
预测物种Ms, Rat, Bov, Pig
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称ENO3
抗原物种Human
抗原KLH-conjugated synthetic peptide corresponding to aa. 237-264 (Center) of Human ENO3.
偶联标记Un-conjugated
別名Skeletal muscle enolase; Muscle-specific enolase; 2-phospho-D-glycerate hydro-lyase; Enolase 3; Beta-enolase; MSE; GSD13; EC 4.2.1.11
应用说明
应用建议
应用推荐稀释比
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Mouse skeletal muscle
属性
形式Liquid
纯化This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
缓冲液PBS and 0.09% (W/V) Sodium azide
抗菌剂0.09% (W/V) Sodium azide
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2027 Human ENO3

Swiss-port # P13929 Human Beta-enolase

基因名称ENO3
全名enolase 3 (beta, muscle)
背景介绍This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]
生物功能Appears to have a function in striated muscle development and regeneration. [UniProt]
细胞定位Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity)
研究领域Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量47 kDa
检测图片 (3)
  • ARG55574 anti-ENO3 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human hepatocarcinoma stained with ARG55574 anti-ENO3 antibody.

  • ARG55574 anti-ENO3 antibody WB image

    Western blot: 35 µg of Mouse skeletal muscle lysate stained with ARG55574 anti-ENO3 antibody.

  • ARG55574 anti-ENO3 antibody FACS image

    Flow Cytometry: HepG2 cells stained with ARG55574 anti-ENO3 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.

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