anti-Enolase 1 / 2 antibody [1G7]

CAT.NO. : ARG57167

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [1G7] recognizes Enolase 1 / 2
反应物种	Hu
应用	FACS, ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1G7
同位型	IgG2a, kappa
靶点名称	Enolase 1 / 2
抗原物种	Human
抗原	Recombinant fragment around aa. 1-434 of Human Enolase1/2
偶联标记	Un-conjugated
別名	MPB1; Plasminogen-binding protein; Alpha-enolase; MBP-1; NNE; PPH; Enolase 1; ENO1L1; Phosphopyruvate hydratase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Non-neural enolase; MPB-1; EC 4.2.1.11

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 2023 Human ENO1 Swiss-port # P06733 Human Alpha-enolase
基因名称	ENO1
全名	enolase 1, (alpha)
背景介绍	This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
生物功能	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor. [UniProt]
预测分子量	47 kDa
翻译后修饰	ISGylated.

检测图片 (4)

ARG57167 anti-Enolase 1/2 antibody [1G7] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57167 anti-Enolase 1/2 antibody [1G7] FACS image
Flow Cytometry: LNCap cell line stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57167 anti-Enolase 1/2 antibody [1G7] WB image
Western blot: 50 ng of 1) ENO1 (Alpha-enolase), 2) ENO2 (Gamma-enolase), and 3) ENO3 (Beta-enolase) recombinant proteins stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:1000.
ARG57167 anti-Enolase 1/2 antibody [1G7] WB image
Western blot: 40 µg of 1) PC3, 2) MCF7, 3) 293T, and 4) HeLa cell lysates stained with ARG57167 anti-Enolase 1/2 antibody [1G7] at 1:1000.

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