anti-eNOS antibody [M221]
![anti-eNOS antibody [M221]](/upload/image/products/ARG23708_IF_1_210_205.jpg)
![anti-eNOS antibody [M221]](/upload/image/products/ARG23708_WB_1.jpg)
![anti-eNOS antibody [M221]](/upload/image/products/ARG23708_IF_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG23708
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [M221] recognizes eNOS |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | M221 |
| 同位型 | IgG1 |
| 靶点名称 | eNOS |
| 抗原物种 | Mouse |
| 抗原 | Recombinant protein around the C-terminus region of Mouse eNOS. This sequence is conserved in Human and Rat eNOS, and has low homology to other NOS family members. |
| 偶联标记 | Un-conjugated |
| 別名 | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
应用说明
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. IHC-P: Antigen Retrieval: Heat mediation was performed in 10 mM Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein A |
| 缓冲液 | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NOS3 |
| 全名 | nitric oxide synthase 3 (endothelial cell) |
| 背景介绍 | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| 生物功能 | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| 预测分子量 | 133 kDa |
| 翻译后修饰 | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. [UniProt] |
检测图片 (2)
ARG23708 anti-eNOS antibody [M221] WB image
Western blot: Human umbilical vein endothelial cells untreated (left) or treated (right) with lambda phosphatase. The blots were stained with ARG23708 anti-eNOS antibody [M221].
ARG23708 anti-eNOS antibody [M221] ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed and NP-40-permeabilized Human umbilical vein endothelial cells. The cells were stained with ARG23708 anti-eNOS antibody [M221].
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