anti-eNOS phospho (Ser1177) antibody
Key features and details
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- 抗原物种:
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Brand:
CAT.NO. : ARG20524
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes eNOS phospho (Ser1177) |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, WB |
| 特异性 | The antibody detects a ~140 kDa band corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells grown normally or treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | eNOS |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide (coupled to carrier protein) around Ser1177 of Human eNOS. This sequence is conserved in rat and mouse eNOS. |
| 偶联标记 | Un-conjugated |
| 別名 | Constitutive NOS; NOS type III; Nitric oxide synthase, endothelial; Endothelial NOS; eNOS; EC-NOS; NOSIII; cNOS; EC 1.14.13.39; ECNOS |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% Sodium azide |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 1 mg/ml BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NOS3 |
| 全名 | nitric oxide synthase 3 (endothelial cell) |
| 背景介绍 | Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. Tyr-657 is phosphorylated by PYK2 in response to fluid shear stress and this phosphorylation leads to attenuation of enzyme activity. |
| 生物功能 | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30247 Phospho eNOS Antibody Duo (Total, pY1177) Related products: eNOS antibodies; eNOS ELISA Kits; eNOS Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody |
| 预测分子量 | 133 kDa |
| 翻译后修饰 | Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. |
检测图片 (1)
ARG20524 anti-eNOS phospho (Ser1177) antibody WB image
Western blot: 1) and 2) calyculin A (100 nM) treated Human umbilical vein endothelial cells for 30 min, 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20524 anti-eNOS phospho (Ser1177) antibody.
参考文献
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Identification and characterization of a novel tetrapeptide from enzymatic hydrolysates of Baijiu byproduct
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Isolation and Characterization of Three Anti-hypertension Peptides from the Mycelia of Ganoderma Lucidum (Agaricomycetes).
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