anti-EpCAM antibody [VU-1D9] (FITC)

产品描述: FITC-conjugated Mouse Monoclonal antibody [VU-1D9] recognizes CD326 / EpCAM
反应物种: Hu
应用: FACS
特异性: The clone VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
宿主: Mouse
克隆: Monoclonal
克隆号: VU-1D9
同位型: IgG1
靶点名称: EpCAM

CAT.NO. : ARG62816

US＄ Please choose

Size:

100 tests

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	FITC-conjugated Mouse Monoclonal antibody [VU-1D9] recognizes CD326 / EpCAM
反应物种	Hu
应用	FACS
特异性	The clone VU-1D9 recognizes an epitope within EGF-like domain I of CD326 / EpCAM, a marker of epithelial lineages. This antibody strongly stains various normal epithelial cells and carcinomas.
宿主	Mouse
克隆	Monoclonal
克隆号	VU-1D9
同位型	IgG1
靶点名称	EpCAM
抗原	Small cell lung carcinoma cell line H69.
偶联标记	FITC
別名	MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326

应用说明

应用建议

应用	推荐稀释比
FACS	20 µl / 10^6 cells

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化说明	The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
缓冲液	PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
抗菌剂	15 mM Sodium azide
稳定剂	0.2% (w/v) high-grade protease free BSA
存放说明	Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4072 Human EPCAM Swiss-port # P16422 Human Epithelial cell adhesion molecule
基因名称	EPCAM
全名	epithelial cell adhesion molecule
背景介绍	EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
生物功能	EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt]
研究领域	Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
预测分子量	35 kDa
翻译后修饰	Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.

检测图片 (1)

ARG62816 anti-EpCAM antibody [VU-1D9] (FITC) FACS image
Flow Cytometry: Human MCF7 (red-filled) and SP2 cells (black-dashed) stained with ARG62816 anti-EpCAM antibody [VU-1D9] (FITC).

参考文献

克隆号文献

Superficial scrapings from breast tumors is a source for biobanking and research purposes.

Ma R et al.
Lab Invest., (2014)