anti-EZH2 antibody
Key features and details
- 产品描述:
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- 应用:
- 宿主:
- 克隆:
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- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes EZH2 |
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| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | EZH2 |
| 抗原物种 | Human |
| 抗原 | Human EZH2 recombinant protein (NP_001190176.1). |
| 偶联标记 | Un-conjugated |
| 別名 | ENX-1; Lysine N-methyltransferase 6; KMT6; ENX1; WVS2; WVS; Enhancer of zeste homolog 2; EZH2b; Histone-lysine N-methyltransferase EZH2; EZH1; EC 2.1.1.43; KMT6A |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 纯化 | Protein A purified |
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| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # Q15910 Human Histone-lysine N-methyltransferase EZH2 Swiss-port # Q61188 Mouse Histone-lysine N-methyltransferase EZH2 |
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| 基因名称 | EZH2 |
| 全名 | enhancer of zeste 2 polycomb repressive complex 2 subunit |
| 背景介绍 | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011] |
| 生物功能 | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription. [From Uniprot] |
| 细胞定位 | Nucleus |
| 产品亮点 | Related Antibody Duos and Panels: ARG30309 Polycomb Complexes Antibody Panel (H3K27me3, EZH2, Ring1A, RNF2) Related products: EZH2 antibodies; EZH2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Polycomb Complexes antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing. Sumoylated. Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex. |
ARG54703 anti-EZH2 antibody WB image
Western blot: 30 µg of U2OS, Jurkat and PC3 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution
ARG54703 anti-EZH2 antibody WB image
Western blot: 30 µg of SW480 and HCT116 cell lysates stained with ARG54703 anti-EZH2 antibody at 1:1000 dilution.
ARG54703 anti-EZH2 antibody ICC/IF image
Immunofluorescence: 293 cells stained with ARG54703 anti-EZH2 antibody. Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
ARG54703 anti-EZH2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue stained with ARG54703 anti-EZH2 antibody.
ARG54703 anti-EZH2 antibody FACS image
Flow Cytometry: HeLa cells stained with ARG54703 anti-EZH2 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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