anti-F-actin antibody [NH3]
![anti-F-actin antibody [NH3]](/upload/image/products/ARG23737_IF_191031-5_210_205.jpg)
![anti-F-actin antibody [NH3]](/upload/image/products/ARG23737_IF_191031-5.jpg)
Key features and details
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- 靶点名称:
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CAT.NO. : ARG23737
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [NH3] recognizes F-actin. |
|---|---|
| 反应物种 | Hu, Ms, Rat, Rb |
| 应用 | ELISA, FACS, ICC/IF, IHC-Fr, IP, WB |
| 特异性 | The clone [NH3] recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody is recommended to use to detect this primary antibody. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | NH3 |
| 同位型 | IgM |
| 靶点名称 | F-actin |
| 抗原物种 | Human |
| 抗原 | Human monocytes and U937 cell line. |
| 偶联标记 | Un-conjugated |
| 別名 | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACTB |
| 背景介绍 | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| 生物功能 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody |
| 预测分子量 | 42 kDa |
检测图片 (1)
ARG23737 anti-F-actin antibody [NH3] ICC/IF image
Immunofluorescence: Mouse cardiac microvascular endothelial cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100/PBS and then stained with ARG23737 anti-F-actin antibody [NH3] at 1:100 dilution.
参考文献
Magnetic stirring with iron oxide nanospinners accretes neurotoxic Aβ42 oligomers into phagocytic clearable plaques for Alzheimer's disease treatment
ICC/IF / Mouse
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