anti-Factor XII Heavy chain antibody [B7C9]
![anti-Factor XII Heavy chain antibody [B7C9]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
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CAT.NO. : ARG54561
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [B7C9] recognizes Factor XII Heavy chain |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, WB |
| 特异性 | This antibody reacts with the heavy chain of human Factor XII (mw~50,000) with KD of 9.8nM and partially blocks Factor XII activity.The specific epitope for this antibody is located within a 20amino acid sequence of 2.5 kD in thesurface binding area of Factor XII. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | B7C9 |
| 同位型 | IgG1 |
| 靶点名称 | Factor XII Heavy chain |
| 抗原物种 | Human |
| 抗原 | Purified human Factor XII. |
| 偶联标记 | Un-conjugated |
| 別名 | Hageman factor; Coagulation factor XII; HAF; EC 3.4.21.38; HAEX; Beta-factor XIIa part 2; HAE3 |
应用说明
| 应用说明 | This antibody may be used in ELISA, Western blot, coagulation assays and for immunopurification of Factor XII. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G-purified |
| 缓冲液 | PBS (pH 7.4) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | F12 |
| 全名 | coagulation factor XII (Hageman factor) |
| 背景介绍 | This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged. [provided by RefSeq, Jul 2008] |
| 生物功能 | Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody |
| 预测分子量 | 68 kDa |
| 翻译后修饰 | Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2. O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. |
参考文献
克隆号文献
Monocytes, neutrophils, and platelets cooperate to initiate and propagate venous thrombosis in mice in vivo.
Transforming growth factor-β1 induces expression of human coagulation factor XII via Smad3 and JNK signaling pathways in human lung fibroblasts.
WB, ICC/IF / Human
Involvement of the contact phase and intrinsic pathway in herpes simplex virus-initiated plasma coagulation.
WB / Human
Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation.
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