anti-FGF acidic antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes FGF acidic
  • 反应物种: Hu, Ms
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: FGF acidic
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human FGF acidic.(MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D)
  • Brand:
CAT.NO. : ARG56708
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes FGF acidic
反应物种Hu, Ms
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称FGF acidic
抗原物种Human
抗原E.coli derived Recombinant Human FGF acidic.
(MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D)
偶联标记Un-conjugated
別名HBGF1; FGF-1; FGF-alpha; GLIO703; ECGFA; ECGFB; ECGF; Endothelial cell growth factor; FGFA; Acidic fibroblast growth factor; Heparin-binding growth factor 1; Fibroblast growth factor 1; HBGF-1; AFGF; aFGF; ECGF-beta
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56817 as a detection antibody
IHC-P0.050 µg/ml - 0.125 µg/ml
Neut1.0 - 2.5 µg/ml (To yield [ND50] of the biological activity of Human FGF - acidic (10.0 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明Please note the application for Neutralizing is lot dependent, if scientist would like to use this antibody for Neutralizing, please contact with arigo.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 14164 Mouse FGF1

GeneID: 2246 Human FGF1

Swiss-port # P05230 Human Fibroblast growth factor 1

Swiss-port # P61148 Mouse Fibroblast growth factor 1

基因名称FGF1
全名fibroblast growth factor 1 (acidic)
背景介绍The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]
生物功能Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
预测分子量17 kDa
翻译后修饰In the nucleus, phosphorylated by PKC/PRKCD.
检测图片 (4)
  • ARG56708 anti-FGF acidic antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal kidney. The recommended ARG56708 anti-FGF acidic antibody concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.

  • ARG56708 anti-FGF acidic antibody WB image

    Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56708 anti-FGF acidic antibody, under reducing conditions.

  • ARG56708 anti-FGF acidic antibody WB image

    Western blot: 250 - 0.24 ng of Human FGF-acidic stained with ARG56708 anti-FGF acidic antibody, under non-reducing conditions.

  • ARG56708 anti-FGF acidic antibody standard curve image

    Sandwich ELISA: ARG56708 anti-FGF acidic antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56817 anti-FGF acidic antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

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