anti-FHL1 / SLIM1 antibody
CAT.NO. : ARG63657
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概述
产品描述 | Goat Polyclonal antibody recognizes FHL1 / SLIM1 |
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反应物种 | Hu, Ms, Rat |
预测物种 | Cow, Dog, Pig |
应用 | FACS, ICC/IF, WB |
特异性 | This antibody is expected to recognise isoforms 2( NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2.Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein. |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | FHL1 / SLIM1 |
抗原物种 | Human |
抗原 | CNKRFVFHNEQVY |
偶联标记 | Un-conjugated |
別名 | FHL1A; FHL1B; KYOT; Four and a half LIM domains protein 1; SLIM; FLH1A; XMPMA; FHL-1; SLIM-1; SLIM1; SLIMMER; Skeletal muscle LIM-protein 1 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. Expression of these family members occurs in a cell- and tissue-specific mode and these proteins are involved in many cellular processes. Mutations in this gene have been found in patients with Emery-Dreifuss muscular dystrophy. Multiple alternately spliced transcript variants which encode different protein isoforms have been described.[provided by RefSeq, Nov 2009] |
研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
预测分子量 | 36 kDa |
检测图片 (2)
ARG63657 anti-FHL1 / SLIM1 antibody ICC/IF image
Immunofluorescence: U2OS stained with ARG63657 anti-FHL1 / SLIM1 antibody at 10 µg/ml dilution.
ARG63657 anti-FHL1 / SLIM1 antibody WB image
Western Blot: Human Muscle lysate (RIPA buffer, 35 µg total protein per lane) stained with ARG63657 anti-FHL1 / SLIM1 antibody at 0.05 µg/ml dilution.
