anti-FOXA2 / HNF3B antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes FOXA2 / HNF3B
  • 反应物种: Hu, Rat
  • 预测物种: Ms
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise both reported isoforms.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: FOXA2 / HNF3B
  • Brand:
CAT.NO. : ARG63339
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes FOXA2 / HNF3B
反应物种Hu, Rat
预测物种Ms
应用IHC-P, WB
特异性This antibody is expected to recognise both reported isoforms.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称FOXA2 / HNF3B
抗原物种Human
抗原C-GVYSRPIMNSS
偶联标记Un-conjugated
別名TCF3B; Hepatocyte nuclear factor 3-beta; Forkhead box protein A2; TCF-3B; HNF-3B; HNF3B; Transcription factor 3B; HNF-3-beta
应用说明
应用建议
应用推荐稀释比
IHC-P4 - 8 µg/ml
WB1 - 3 µg/ml
应用说明IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 25099 Rat FOXA2

GeneID: 3170 Human FOXA2

Swiss-port # P32182 Rat Hepatocyte nuclear factor 3-beta

Swiss-port # Q9Y261 Human Hepatocyte nuclear factor 3-beta

背景介绍This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
研究领域Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody
预测分子量48 kDa
翻译后修饰Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
检测图片 (3)
  • ARG63339 anti-FOXA2 / HNF3B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63339 anti-FOXA2 / HNF3B antibody at 3.8 µg/ml dilution.

  • ARG63339 anti-FOXA2 / HNF3B antibody WB image

    Western blot: 35 µg of Human liver lysate (in RIPA buffer) stained with ARG63339 anti-FOXA2 / HNF3B antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63339 anti-FOXA2 / HNF3B antibody WB image

    Western blot: 35 µg of Rat lung lysate (in RIPA buffer) stained with ARG63339 anti-FOXA2 / HNF3B antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

参考文献

CD44 Promotes Breast Cancer Metastasis through AKT-Mediated Downregulation of Nuclear FOXA2

WB / Human

Anupama Vadhan et al.
Biomedicines,  (2022)

publication_link

 

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