anti-Frizzled 2 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Frizzled 2
  • 反应物种: Hu
  • 预测物种: Ms, Rat
  • 应用: ICC/IF, IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Frizzled 2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG64623
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Frizzled 2
反应物种Hu
预测物种Ms, Rat
应用ICC/IF, IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Frizzled 2
抗原物种Human
抗原RYATLEHPFHCPR
偶联标记Un-conjugated
別名fzE2; FzE2; Fz2; Frizzled-2; hFz2; Fz-2; fz-2
应用说明
应用建议
应用推荐稀释比
ICC/IF10 µg/ml
IHC-P2 - 5 µg/ml
WB0.03 - 0.1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 2535 Human FZD2

Swiss-port # Q14332 Human Frizzled-2

背景介绍This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. This gene encodes a protein that is coupled to the beta-catenin canonical signaling pathway. Competition between the wingless-type MMTV integration site family, member 3A and wingless-type MMTV integration site family, member 5A gene products for binding of this protein is thought to regulate the beta-catenin-dependent and -independent pathways. [provided by RefSeq, Dec 2010]
研究领域Cancer antibody; Developmental Biology antibody; Signaling Transduction antibody
预测分子量64 kDa
翻译后修饰Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
检测图片 (4)
  • ARG64623 anti-Frizzled 2 antibody ICC/IF image

    Immunofluorescence: Paraformaldehyde fixed U2OS cells permeabilized with 0.15% Triton. Cells were stained with ARG64623 anti-Frizzled 2 antibody (green) at 10 µg/ml dilution for 1 hour. Phalloidin (red) for Actin filaments staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.

  • ARG64623 anti-Frizzled 2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64623 anti-Frizzled 2 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG64623 anti-Frizzled 2 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Thyroid Gland. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64623 anti-Frizzled 2 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG64623 anti-Frizzled 2 antibody WB image

    Western blot: 35 µg of Human heart (A) and Human placenta (B) (in RIPA buffer) stained with ARG64623 anti-Frizzled 2 antibody at 0.03 µg/ml (A) and 0.1 µg/ml (B) dilutions and incubated at RT for 1 hour.

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