anti-FZR1 / CDH1 antibody [DH01 (DCS-266)]
![anti-FZR1 / CDH1 antibody [DH01 (DCS-266)]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
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CAT.NO. : ARG62433
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [DH01 (DCS-266)] recognizes FZR1 / CDH1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | DH01 (DCS-266) |
| 同位型 | IgG1 |
| 靶点名称 | FZR1 / CDH1 |
| 抗原物种 | Human |
| 抗原 | Recombinant full length protein (Human) |
| 偶联标记 | Un-conjugated |
| 別名 | CDC20-like protein 1; hCDH1; FZR2; FZR; Fizzy-related protein homolog; HCDH1; HCDH; Fzr; CDH1; CDC20C; Cdh1/Hct1 homolog |
应用说明
| 应用说明 | IP: 2 µg/ml WB: 2 µg/ml FACS: 1: 10 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FZR1 |
| 全名 | fizzy/cell division cycle 20 related 1 |
| 生物功能 | Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | Acetylated. Deacetylated by SIRT2 at Lys-69 and Lys-159; deacetylation enhances the interaction of FZR1 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C). Phosphorylated during mitosis, probably by maturation promoting factor (MPF), leading to its dissociation of the APC/C. Following DNA damage, it is dephosphorylated by CDC14B in G2 phase, leading to its reassociation with the APC/C, and allowing an efficient G2 DNA damage checkpoint. Phosphorylated by MAK. |
参考文献
克隆号文献
WEE1 murine deficiency induces hyper-activation of APC/C and results in genomic instability and carcinogenesis.
6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27.
IP / Human
A proteomic chronology of gene expression through the cell cycle in human myeloid leukemia cells.
WB / Human
Uncovering the role of APC-Cdh1 in generating the dynamics of S-phase onset.
Checkpoint kinase 1 is essential for meiotic cell cycle regulation in mouse oocytes.
WB / Mouse
Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes.
WB, IHC-P / Mouse
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