anti-Galectin 3 antibody (Biotin)

Key features and details

  • 产品描述: Biotin-conjugated Rabbit Polyclonal antibody recognizes Galectin 3
  • 反应物种: Hu
  • 应用: ELISA, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Galectin 3
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human Galectin-3.(ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI)
  • Brand:
CAT.NO. : ARG56797
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Product Details
概述
产品描述Biotin-conjugated Rabbit Polyclonal antibody recognizes Galectin 3
反应物种Hu
应用ELISA, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Galectin 3
抗原物种Human
抗原E.coli derived Recombinant Human Galectin-3.
(ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYHGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSAPGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI)
偶联标记Biotin
別名Laminin-binding protein; Gal-3; L-31; GALBP; Galactoside-binding protein; MAC2; GAL3; GALIG; Mac-2 antigen; CBP 35; Galectin-3; CBP35; Galactose-specific lectin 3; IgE-binding protein; L31; 35 kDa lectin; Carbohydrate-binding protein 35; Lectin L-29
应用说明
应用建议
应用推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG56688 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified by affinity chromatography.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3958 Human LGALS3

Swiss-port # P17931 Human Galectin-3

基因名称LGALS3
全名lectin, galactoside-binding, soluble, 3
背景介绍This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014]
生物功能Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. [UniProt]
预测分子量26 kDa
检测图片 (4)
  • ARG56797 anti-Galectin 3 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56797 anti-Galectin 3 antibody (Biotin), under reducing conditions.

  • ARG56797 anti-Galectin 3 antibody (Biotin) standard curve image

    Direct ELISA: ARG56797 anti-Galectin 3 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density.

  • ARG56797 anti-Galectin 3 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human Galectin-3 stained with ARG56797 anti-Galectin 3 antibody (Biotin), under non-reducing conditions.

  • ARG56797 anti-Galectin 3 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG56797 anti-Galectin 3 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG56688 anti-Galectin 3 antibody as a capture antibody. Results of a typical standard run with optical density.

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