Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00](/upload/image/products/Fig-RM224-Gamma-H2AX-Lung-Cancer-with-Radiotherapy.jpg)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00](/upload/image/products/Fig1_WB_gH2AX_e.png)
![Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody; Histone Gamma-H2A.X (Phospho S139) [Clone RM224], 31-1104-00](/upload/image/products/Fig2_ICC_gH2AX_e.png)
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Product Details
| Product Name | Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody, Clone RM224 |
| Product Description | Rabbit monoclonal to Gamma H2AX (phospho-Ser139) |
| Catalog No. | 31-1104-00 |
| Clone Name | RM224 |
| Product Data Sheet | Anti-Gamma H2AX (phospho-Ser139) Rabbit Monoclonal Antibody Data Sheet RM224 |
| Specificity | RM224 reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) Immunohistochemistry (IHC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt. |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 μg/mL – 2 μg/mL; ELISA: 0.2 μg/mL - 1 μg/mL; Multiplex: 0.1 μg/mL – 1 μg/mL; ICC: 0.5 μg/mL - 2 μg/mL. |
| Keywords | |
| . | |
 | Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 ug/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells. |
 | Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
| Immunohistochemical staining of FFPE Lung cancer with radiotherapy tissues, Using anti-gamma-H2AX [RM224] rabbit monoclonal antibodies. |
 | IHC Staining Comparison of FFPE normal lung, Lung cancer without chemotherapy & radiotherapy, Lung cancer with chemotherapy, Lung cancer with radiotherapy tissues, Using anti-DNA-PKcs (total) [RM505], anti-phospho-DNA-PKcs (Ser2056) [RM500], anti-gamma-H2AX [RM224] rabbit monoclonal antibodies. |
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