anti-GATA3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
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- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG55132
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody recognizes GATA3 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1259CT707.136.102 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | GATA3 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human GATA3. |
| 偶联标记 | Un-conjugated |
| 別名 | Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3; HDR; HDRS |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HL-60 |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P23771 Human Trans-acting T-cell-specific transcription factor GATA-3 |
|---|---|
| 基因名称 | GATA3 |
| 全名 | GATA binding protein 3 |
| 背景介绍 | This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009] |
| 生物功能 | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. [UniProt] |
| 细胞定位 | Nucleus. |
| 研究领域 | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Immune System antibody |
| 预测分子量 | 48 kDa |
检测图片 (3)
ARG55132 anti-GATA3 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG55132 anti-GATA3 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
ARG55132 anti-GATA3 antibody WB image
Western blot: 35 µg of HL-60 cell lysate stained with ARG55132 anti-GATA3 antibody at 1:1000 dilution.
ARG55132 anti-GATA3 antibody FACS image
Flow Cytometry: MCF7 cells stained with ARG55132 anti-GATA3 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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