anti-GFAP antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)
  • 反应物种: Hu, Ms, Rat
  • 预测物种: Dog
  • 应用: IHC-Fr, IHC-P, WB
  • 特异性: GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: GFAP
  • Brand:
CAT.NO. : ARG64066
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP)
反应物种Hu, Ms, Rat
预测物种Dog
应用IHC-Fr, IHC-P, WB
特异性GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称GFAP
抗原物种Human
抗原C-DGEVIKESKQEHKD
偶联标记Un-conjugated
別名Glial fibrillary acidic protein; ALXDRD; GFAP
应用说明
应用建议
应用推荐稀释比
IHC-Fr0.01 µg/ml
IHC-PAssay - dependent
WB0.003 - 0.01 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 14580 Mouse GFAP

GeneID: 24387 Rat GFAP

GeneID: 2670 Human GFAP

全名glial fibrillary acidic protein
背景介绍GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
生物功能GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt]
产品亮点Related Antibody Duos and Panels:
ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit)
Related products:
GFAP antibodies; GFAP Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
研究领域Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
预测分子量50 kDa
翻译后修饰Phosphorylated by PKN1.
检测图片 (4)
  • ARG64066 anti-GFAP antibody WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG64066 anti-GFAP antibody at 1:5000 dilution.

  • ARG64066 anti-GFAP antibody IHC-Fr image

    Immunohistochemistry: PFA-perfused cryosection of Human hypothalamus tissue stained with ARG64066 anti-GFAP antibody at 0.01 µg/ml dilution.

  • ARG64066 anti-GFAP antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64066 anti-GFAP antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG64066 anti-GFAP antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64066 anti-GFAP antibody at 2 µg/ml dilution, followed by HRP-staining.

参考文献

Acupuncture promotes neurological recovery and regulates lymphatic function after acute inflammatory nerve root injury

IHC-Fr / Human

Jie Wang et al.
Heliyon.,  (2024)

publication_link

 

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