anti-GST antibody [S-tag-05]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [S-tag-05] recognizes GST
  • 反应物种: Other
  • 应用: IP, WB
  • 特异性: The clone S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: S-tag-05
  • 同位型: IgG2b
  • 靶点名称: GST
  • Brand:
CAT.NO. : ARG62998
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Product Details
概述
产品描述Mouse Monoclonal antibody [S-tag-05] recognizes GST
反应物种Other
应用IP, WB
特异性The clone S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibody is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.
宿主Mouse
克隆Monoclonal
克隆号S-tag-05
同位型IgG2b
靶点名称GST
抗原物种Others
抗原GST fusion protein
偶联标记Un-conjugated
別名Glutathione S-transferase kappa 1; hGSTK1; GST; GST class-kappa; GST13-13; EC 2.5.1.18; Glutathione S-transferase subunit 13; GST13; GST 13-13; GSTK1-1
应用说明
应用建议
应用推荐稀释比
IPAssay-dependent
WB1 µg/ml
应用说明WB: Under reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照WB: GST fusion protein.
属性
形式Liquid
纯化Purified from hybridoma culture supernatant by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
基因名称GSTK1
全名glutathione S-transferase kappa 1
背景介绍The GST (Glutathione-S-Transferase) is a 26kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.

Antibodies against GST will detect the expressed GST protein or a GST-fusion protein, and can be utilized for immuno-purification of GST-fusion proteins.
生物功能Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB). [UniProt]
研究领域Cell Biology and Cellular Response antibody; Controls and Markers antibody; Metabolism antibody; Signaling Transduction antibody
检测图片 (1)
  • ARG62998 anti-GST antibody [S-tag-05] WB image

    Western blot: Affinity purified GST from bacterial cell lysate stained with ARG62998 anti-GST antibody [S-tag-05].

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