anti-GST tag antibody [SQab1886]

Key features and details

产品描述: Recombinant Rabbit Monoclonal antibody [SQab1886] recognizes GST tag
反应物种: Other
应用: FACS, ICC/IF, IP, WB
宿主: Rabbit
克隆: Monoclonal
克隆号: SQab1886
同位型: IgG
靶点名称: GST tag
抗原物种: Others

Brand:

CAT.NO. : ARG66338

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Size:

100 μl 50 μl 20 μl

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Recombinant Rabbit Monoclonal antibody [SQab1886] recognizes GST tag
反应物种	Other
应用	FACS, ICC/IF, IP, WB
宿主	Rabbit
克隆	Monoclonal
克隆号	SQab1886
同位型	IgG
靶点名称	GST tag
抗原物种	Others
抗原	Recombinant full length protein within aa. 1-218 of Schistosoma japonicum GST.
偶联标记	Un-conjugated

应用说明

应用建议

应用	推荐稀释比
FACS	1:800 - 1:2000
ICC/IF	1:10000 - 1:40000
IP	1:50
WB	1:2000 - 1:5000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
抗菌剂	0.01% Sodium azide
稳定剂	40% Glycerol and 0.05% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

检测图片 (6)

ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image
Immunofluorescence: 293 cells transfected with N-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG66338 anti-GST tag antibody [SQab1886] FACS image
Flow Cytometry: 293 cells transfected with C-terminal (red) and N-terminal (blue) GST tagged gene. The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2,000 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control. .
ARG66338 anti-GST tag antibody [SQab1886] WB image
Western blot: 1) 2 µg of 293 cell lysates transfected with N-terminal GST tagged gene, 2) 2 µg of 293 cell lysates transfected with C-terminal GST tagged gene, 3) 10 µg of Mock 293 cell lysates stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:10000 dilution, and 4) 20 ng of two fusion proteins, one (45 kDa) with GST tag on C-terminal, the other (83 kDa) with GST tag on N-terminal stained with ARG66338 anti-GST tag antibody [SQab1886] at 1:2000 dilution.
ARG66338 anti-GST tag antibody [SQab1886] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with C-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with C-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with C-terminal GST tagged gene, and 3) 293 whole cell lysate transfected with C-terminal GST tagged gene, 2 µg (input).
ARG66338 anti-GST tag antibody [SQab1886] ICC/IF image
Immunofluorescence: 293 cells transfected with C-terminal GST tagged gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66338 anti-GST tag antibody [SQab1886] (red) at 1:40,000 and 4°C. DAPI (blue) was used as the nuclear counter stain.
ARG66338 anti-GST tag antibody [SQab1886] IP image
Immunoprecipitation: 0.2 mg of 293 whole cell lysates transfected with N-terminal GST tagged gene immunoprecipitated (1:50) and stained with ARG66338 anti-GST tag antibody [SQab1886]. 1) ARG66338 IP in 293 whole cell lysates transfected with N-terminal GST tagged gene, 2) PBS instead of ARG66338 in 293 whole cell lysates transfected with N-terminal GST tagged gene, and 3) 293 whole cell lysates transfected with N-terminal GST tagged gene, 2 µg (input).