anti-H11 / Hsp22 antibody
产品描述 | Goat Polyclonal antibody recognizes H11 / Hsp22 |
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反应物种 | Hu |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | H11 / Hsp 22 |
抗原物种 | Human |
抗原 | NELPQDSQEVTCT |
偶联标记 | Un-conjugated |
別名 | E2-induced gene 1 protein; E2IG1; Protein kinase H11; HMN2; HMN2A; H11; Alpha-crystallin C chain; CMT2L; Small stress protein-like protein HSP22; HspB8; DHMN2; HSP22; Heat shock protein beta-8 |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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背景介绍 | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. [provided by RefSeq, Jul 2008] |
研究领域 | Neuroscience antibody; Signaling Transduction antibody |
预测分子量 | 22 kDa |
ARG63276 anti-H11 / Hsp22 antibody WB image
Western blot: Human muscle lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63276 anti-H11 / Hsp22 antibody at 1 µg/ml dilution.
ARG63276 anti-H11 / Hsp22 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human adrenal gland tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63276 anti-H11 / Hsp22 antibody at 10 µg/ml dilution followed by AP-staining.
ARG63276 anti-H11 / Hsp22 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63276 anti-H11 / Hsp22 antibody at 10 µg/ml dilution followed by AP-staining.
