anti-HA tag antibody [HA.C5]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [HA.C5] recognizes HA tag
  • 反应物种: Other
  • 应用: Dot, ELISA, FACS, ICC/IF, IHC, IP, WB
  • 特异性: Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: HA.C5
  • 同位型: IgG3
  • 靶点名称: HA tag
  • Brand:
CAT.NO. : ARG62338
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Product Details
概述
产品描述Mouse Monoclonal antibody [HA.C5] recognizes HA tag
反应物种Other
应用Dot, ELISA, FACS, ICC/IF, IHC, IP, WB
特异性Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins
宿主Mouse
克隆Monoclonal
克隆号HA.C5
同位型IgG3
靶点名称HA tag
抗原物种Others
抗原YPYDVPDYA (HA) synthetic peptide conjugated to KLH.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
DotAssay-dependent
ELISAAssay-dependent
FACSAssay-dependent
ICC/IF1:500 - 1:2000
IHC1:500 - 1:2000
IP1:50-1:200
WB1:1000 - 1:3000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Protein A Purified
纯化说明Protein A affinity chromatography from mouse ascites fluid.
缓冲液10mM PBS (pH 7.2) and 0.05% Sodium azide.
抗菌剂0.05% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
背景介绍Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Duo to HA-tag is from influenza virus and not be found in nomal anamils, it is useful for the protein functional analysis when the specific antibodies for the protein-of-interest are unavailable.
产品亮点Related products:
HA tag antibodies; Anti-Mouse IgG secondary antibodies;
研究领域Controls and Markers antibody; Gene Regulation antibody
检测图片 (1)
  • ARG62338 anti-HA tag antibody [HA.C5] WB image

    Western blot: 10 µg of 293T and 293T + myc-HA-mCherry cell lysates stained with ARG62338 anti-HA tag antibody [HA.C5] at 1:1000 dilutioin.

参考文献

Dual protection by Bcp1 and Rkm1 ensures incorporation of uL14 into pre-60S ribosomal subunits

WB / Yeast

Min-Chi Yeh et al.
J Cell Biol.,  (2024)

publication_link

 

hr_line

Translation initiation factor eIF4G1 modulates assembly of the polypeptide exit tunnel region in yeast ribosome biogenesis

WB / Yeast

Yun-Ting Tseng et al.
J Cell Sci,  (2022)

publication_link

 

hr_line

SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK

IP / 

Yang Yang et al.
Cell Death Differ.,  (2022)

publication_link

 

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