Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody [Clone RMH02], 54-1191-00
![Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody [Clone RMH02], 54-1191-00](/upload/image/products/Fig1_WB_HuHAtag.png)
![Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody [Clone RMH02], 54-1191-00](/upload/image/products/Fig2_ELISAcapture_HuHAtag.png)
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CAT.NO. : 54-1191-00
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Product Details
Product Details
| Product Name | Anti-HA-Tag Rabbit/Human ChimericMonoclonal Antibody, Clone RMH02 |
| Product Description | Rabbit/Human Chimeric monoclonal to HA-Tag This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HA-Tag monoclonal antibody (RM305), with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody. |
| Catalog No. | 54-1191-00 |
| Clone Name | RMH02 |
| Product Data Sheet | Anti-HA-Tag Rabbit/Human Chimeric Monoclonal Antibody Data Sheet RMH02 |
| Specificity | RMH02 reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein. |
| Immunogen | A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106). |
| Application | Western Blot ELISA (Capturer or Detector) |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit/Human Chimeric Rabbit Fv/ Human IgG1 CH and Cκ |
| Usage | ELISA: Capturer: 0. 5 µg/mL – 2 µg/mL; Detector: 0.01 µg/mL – 0.5 µg/mL; Western Blot: 0.1 µg/mL – 1 µg/mL. |
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Product Images
 | Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with 50 µL/well of chimeric human anti-HA- Tag antibody Clone RMH02 (1 µg/mL) as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody. |
 | Western blot of 293T cells transfected (+) or non- transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using anti-HA-tag rabbit monoclonal antibody (clone RM305) at 0.1 µg/mL, followed by a HRP conjugated anti-Human IgG secondary antibody. |
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