anti-HDAC2 antibody [3B1]
![anti-HDAC2 antibody [3B1]](/upload/image/products/ARG58293_WB_1_210_205.jpg)
![anti-HDAC2 antibody [3B1]](/upload/image/products/ARG58293_IF_1.jpg)
![anti-HDAC2 antibody [3B1]](/upload/image/products/ARG58293_FACS_1.jpg)
![anti-HDAC2 antibody [3B1]](/upload/image/products/ARG58293_WB_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG58293
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [3B1] recognizes HDAC2 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3B1 |
| 同位型 | IgG2b, kappa |
| 靶点名称 | HDAC2 |
| 抗原物种 | Human |
| 抗原 | Recombinant protein corresponding to aa. 1-488 of Human HDAC2. |
| 偶联标记 | Un-conjugated |
| 別名 | Histone deacetylase 2; EC 3.5.1.98; HD2; YAF1; RPD3 |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HDAC2 |
| 全名 | histone deacetylase 2 |
| 背景介绍 | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
| 生物功能 | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. S-Nitrosylation interferes with its interaction with MTA1 (By similarity). [UniProt] |
检测图片 (3)
ARG58293 anti-HDAC2 antibody [3B1] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG58293 anti-HDAC2 antibody [3B1] (green) at 1:100 dilution. DAPI was stained the cell nucleus (blue).
ARG58293 anti-HDAC2 antibody [3B1] FACS image
Flow Cytometry: A549 cells stained with ARG58293 anti-HDAC2 antibody [3B1] at 2-5 µg / 10^6cells (red). A Goat-anti Mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).
ARG58293 anti-HDAC2 antibody [3B1] WB image
Western blot: 50 ng of Recombinant Human HDAC2 protein stained with ARG58293 anti-HDAC2 antibody [3B1] at 1:1000 dilution.
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