anti-HDGF antibody [SQab1746]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [SQab1746] recognizes HDGF |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, FACS, ICC/IF, IHC, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SQab1746 |
| 同位型 | IgG1 |
| 靶点名称 | HDGF |
| 抗原物种 | Human |
| 抗原 | Recombinant Human HDGF protein. |
| 偶联标记 | Un-conjugated |
| 別名 | HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 缓冲液 | PBS (pH 7.4) and 0.01% Thimerosal. |
| 抗菌剂 | 0.01% Thimerosal |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | HDGF |
| 全名 | hepatoma-derived growth factor |
| 背景介绍 | HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016] |
| 生物功能 | HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618). Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719). Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt] |
| 产品亮点 | Related products: HDGF antibodies; HDGF ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: The most sensitive human HDGF ELISA kit The role of HDGF in tumor angiogenesis |
| 研究领域 | Cancer; Growth factor |
| 预测分子量 | 27 kDa |
| 翻译后修饰 | Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. [UniProt] |
ARG66283 anti-HDGF antibody [SQab1746] ICC/IF image
Immunofluorescence: Cells (A549 and MCF-7) were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66283 anti-HDGF antibody [SQab1746] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).
ARG66283 anti-HDGF antibody [SQab1746] FACS image
Flow Cytometry: 293T cells stained with ARG66283 anti-HDGF antibody [SQab1746] at 1 µg/ml (red) and without antibody control (black).
ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 15 µg of 293T cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
ARG66283 anti-HDGF antibody [SQab1746] ELISA image
ELISA: Titration curve of ARG66283 anti-HDGF antibody [SQab1746] in ELISA. Red: HDGF; Black: BSA (negative control).
ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 20 µg of HuH7 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 20 µg of 3T3 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 50 µg Mouse liver and Rat liver lysates stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
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