anti-Hexokinase (pan) antibody [4D7]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [4D7] recognizes Hexokinase (pan)
  • 反应物种: Hu
  • 应用: ICC/IF, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4D7
  • 同位型: IgG2a, kappa
  • 靶点名称: Hexokinase (pan)
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56938
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Product Details
概述
产品描述Mouse Monoclonal antibody [4D7] recognizes Hexokinase (pan)
反应物种Hu
应用ICC/IF, WB
宿主Mouse
克隆Monoclonal
克隆号4D7
同位型IgG2a, kappa
靶点名称Hexokinase (pan)
抗原物种Human
抗原Recombinant fragment around aa. 1-917 of Human Hexokinase (pan).
偶联标记Un-conjugated
別名Brain form hexokinase; HK1-tc; HK1-tb; HK1-ta; HMSNR; Hexokinase-1; Hexokinase type I; HK I; HKI; HXK1; EC 2.7.1.1; HKD
应用说明
应用建议
应用推荐稀释比
ICC/IFAssay-dependent
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3098 Human HK1

Swiss-port # P19367 Human Hexokinase-1

基因名称HK1
全名hexokinase 1
背景介绍Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]
预测分子量102 kDa
检测图片 (4)
  • ARG56938 anti-Hexokinase (pan) antibody [4D7] ICC/IF image

    Immunoflorescense: HeLa cells stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000 (Green).

    Hoechst 33342 (Blue) for nucleus staining.

  • ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image

    Western blot: 20 ng of 1) Hexokinase 1 recombinant protein, 2) Hexokinase 2 recombinant protein, 3) Hexokinase 3 recombinant protein, 4) Hexokinase 4 recombinant protein stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.

  • ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image

    Western blot: 40 µg of 1) HepG2 cell lysate, 2) HeLa cell lysate, 3) Jurkat cell lysate, 4) K562 cell lysate stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.

  • ARG56938 anti-Hexokinase (pan) antibody [4D7] WB image

    Western blot: 40 µg of Brain tissue lysate stained with ARG56938 anti-Hexokinase (pan) antibody [4D7] at 1:1000.

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