Anti-HIF-1-alpha Rabbit Monoclonal Antibody [Clone RM242], 31-1121-00
![Anti-HIF-1-alpha Rabbit Monoclonal Antibody [Clone RM242], 31-1121-00](/upload/image/products/RM242_Fig1_IHC.png)
![Anti-HIF-1-alpha Rabbit Monoclonal Antibody [Clone RM242], 31-1121-00](/upload/image/products/Fig2_WB_HIF1a.png)
Key features and details
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CAT.NO. : 31-1121-00
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Product Details
Product Details
| Product Name | Anti-HIF-1-alpha Rabbit Monoclonal Antibody, Clone RM242 |
| Product Description | Rabbit monoclonal to HIF-1-alpha |
| Catalog No. | 31-1121-00 |
| Clone Name | RM242 |
| Product Data Sheet | Anti-HIF-1-alpha Rabbit Monoclonal Antibody Data Sheet RM242 |
| Specificity | RM242 reacts to human HIF-1-alpha (Hypoxia-inducible factor 1-alpha). |
| Immunogen | A peptide corresponding to Hypoxia-inducible factor 1-alpha |
| Application | Immunohistochemistry (IHC) Western Blot (WB) |
| Species Reactivity | Human; Mouse |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 μL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Immunohistochemistry (IHC): 1:500-1:1000 dilution; Western Blot (WB): 1:1000-1:2000 dilution. |
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Product Images
| Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242 at a 1:1000 dilution. |
 | Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt (II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expression was induced by CoCl2 in Jurkat cells. |
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