Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240; Histone H3.3 G34R Mutant; 31-1120-00-S; 31-1120-00-L; 31-1120-02
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Product Details
| Product Name | Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody, Clone RM240 |
| Product Description | Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant |
| Catalog No. | 31-1120-00-S; 31-1120-00-L; 31-1120-02 |
| Clone Name | RM240 |
| Product Data Sheet | Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240 Biotinylated Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240 |
| Specificity | RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 . |
| Immunogen | A peptide corresponding to Histone H3.3 G34R mutant |
| Application | Western Blot ELISA Immunohistochemistry Chromatin IP |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µL; 400 µL; Biotinylated 50 µL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1:200 – 1:1000; ELISA: 1:250 – 1:2500; IHC: 1:100 – 1:500. |
| References for Histone H3.3 G34R Mutant antibody [RM240] | Nambiraja A et al C19MC amplification and expression of Lin28A and Olig2 in the classification of embryonal tumors of the central nervous system: A 14-year retrospective study from a tertiary care center. Childs Nerv Syst. (2021), 10.1007/s00381-020-04973-0. IHC; Human. Read more (PubMed: 33236184) Esteve-Codina A et al RNA sequencing and Immunohistochemistry Reveal ZFN7 as a Stronger Marker of Survival than Molecular Subtypes in G-CIMP-negative Glioblastoma. Clin Cancer Res. (2021), 10.1158/1078-0432.CCR-20-2141. IHC; Human. Read more (PubMed: 33106291) Bressa RB et al Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants. Cell Stem Cell. (2021), 10.1016/j.stem.2021.01.016. IHC; Human. Read more (PubMed: 33631116) Minasi S et al Alternative lengthening of telomeres in molecular subgroups of paediatric high-grade glioma. Childs Nerc Syst. (2020), 10.1007/s00381-020-04933-8. IHC; Human. Read more (PubMed: 33128602) Pathania M et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017) 10.1016/j.ccell.2017.09.014. IHC-P; Human. Read more (PubMed: 29107533) Yamamoto H et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017) 10.1016/j.humpath.2017.11.020. IHC; Human. Read more (PubMed: 29241742) |
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 | Western Blot analysis of cell lysates prepared from 293T, transfected with a DNA construct encoding G34R mutant or wild type proteins of Histone H3.3,using anti-Histone H3.3 G34R, Clone RM240. |
 | IHC of clinically validated FFPE Glioblastoma tumor tissues with H3.3 G34R expression, using anti-histoneH3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validated to be Positive for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCL Institute of Neurology, London, United Kingdom |
 | IHC of clinically validated FFPE Glioblastoma tumor tissues, using anti-histone H3.3 G34R rabbit monoclonal antibody, Clone RM240. Clinically validatedto be Negative for H3.3 G34R Mutation. Image courtesy of Dr. Sebastian Brandner, UCLInstitute of Neurology, London, United Kingdom |
 | ICC staining of primary human cell lines expressing WT and H3.3 G34R Mutation. Image courtesy of Dr. Nada Jabado, McGill University, Montreal, Quebec, Canada. |
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