Anti-Histone H3.3 Rabbit Monoclonal Antibody [Clone RM190], 31-1058-00;31-1058-00-S
![Anti-Histone H3.3 Rabbit Monoclonal Antibody [Clone RM190], 31-1058-00;31-1058-00-S](/upload/image/products/RM190_Fig2.png)
![Anti-Histone H3.3 Rabbit Monoclonal Antibody [Clone RM190], 31-1058-00;31-1058-00-S](/upload/image/products/RM190_Fig1_E.png)
Key features and details
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CAT.NO. : 31-1058-00
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Product Details
Product Details
| Product Name | Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190 |
| Product Description | Rabbit monoclonal to Histone H3.3 |
| Catalog No. | 31-1058-00 |
| Clone Name | RM190 |
| Product Data Sheet | Anti-Histone H3.3 Rabbit Monoclonal Antibody Data Sheet RM190 |
| Specificity | RM190 reacts to Histone H3.3, independent of post-translational modifications. No cross reactivity with Histone H3.1 or other histone proteins. |
| Immunogen | A peptide corresponding to human Histone H3.3. |
| Application | Western Blot (WB) ELISA Multiplex Immunofluorescence (IF) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL – 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.05 µg/mL – 0.5 µg/mL. |
| Keywords | |
| References for Histone H3.3 Rabbit Monoclonal Antibody [RM190] | Hinchcliffe EH et al. Chromosome missegregation during anaphase triggers p53 cell cycle arrest through histone H3.3 Ser31 phosphorylation Nat Cell Biol. (2016) 10.1038/ncb3348. IF; Human, Monkey. Read more (PubMed: 27136267) |
Product Images
 | RM190 reacts specifically to Histone H3.3.No cross reactivity with Histone H3.1. |
 | Western Blot of HeLa whole cell lysate, recombinant Histone H3.3 and Histone H3.1 proteins, using RM190 at 1 µg/mL, showed a band of histone H3.3 in HeLa cells and recombinant H3.3 protein, and no cross activity with Histone H3.1. |
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