Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [Clone RM186]; Histone H3 (Unmodified Lys4); 31-1086-00; 31-1086-00-S
![Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [Clone RM186]; Histone H3 (Unmodified Lys4); 31-1086-00; 31-1086-00-S](/upload/image/products/Fig-3_ICC_RM186.png)
![Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [Clone RM186]; Histone H3 (Unmodified Lys4); 31-1086-00; 31-1086-00-S](/upload/image/products/Fig-1_RM186_e.png)
![Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [Clone RM186]; Histone H3 (Unmodified Lys4); 31-1086-00; 31-1086-00-S](/upload/image/products/Fig-2_RM186_e.png)
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CAT.NO. : 31-1086-00
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Product Details
Product Details
| Product Name | Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone RM186 |
| Product Description | Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4) |
| Catalog No. | 31-1086-00 |
| Clone Name | RM186 |
| Product Data Sheet | Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186 |
| Specificity | RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3. |
| Immunogen | An unmodified peptide corresponding to the N-terminus of Histone H3. |
| Application | Western Blot (WB) ELISA Multiplex Immunocytochemistry (ICC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1 µg/mL – 2 µg/mL; ELISA: 0.5 µg/mL - 1 µg/mL; Multiplex: 0.5 µg/mL – 2 µg/mL; Immunocytochemistry: 0.5 µg/mL - 2 µg/mL. |
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Product Images
 | RM186 specifically recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated, mono-methylated, dimethylated, or trimethylated Lys4. The antibody binding specificity allows for modifications of Arg2 or Thr3 in histone H3. |
 | Western Blot of acid extracts of HeLa cells treated with sodium butyrate (HeLa+NaBu), treated with Nocodazole (HeLa+Noc), or non-treated; and recombinant histone H3.1 protein, using RM186 at 1 µg/mL, showed a band of histone H3 with unmodified Lys4. |
 | Immunocytochemical staining of HeLa cells treated with sodium butyrate, using anti-Acetyl-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody, Clone RM186 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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