anti-HLA E antibody [MEM-E/02]

Key features and details

产品描述: Mouse Monoclonal antibody [MEM-E/02] recognizes HLA E
反应物种: Hu, NHuPrm
应用: IHC-P, WB
特异性: The clone MEM-E/02 specifically reacts with denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
宿主: Mouse
克隆: Monoclonal
克隆号: MEM-E/02
同位型: IgG1
靶点名称: HLA E

Brand:

CAT.NO. : ARG63017

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Size:

100 μg

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [MEM-E/02] recognizes HLA E
反应物种	Hu, NHuPrm
应用	IHC-P, WB
特异性	The clone MEM-E/02 specifically reacts with denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
宿主	Mouse
克隆	Monoclonal
克隆号	MEM-E/02
同位型	IgG1
靶点名称	HLA E
抗原物种	Human
抗原	Recombinant human HLA-E denaturated heavy chain.
偶联标记	Un-conjugated
別名	MHC class I antigen E; QA1; EA2.1; HLA-6.2; EA1.2; MHC; HLA class I histocompatibility antigen, alpha chain E

应用说明

应用建议

应用	推荐稀释比
IHC-P	5 - 10 µg/ml
WB	Assay-dependent

应用说明

IHC-P: Pretreatment: Heat antigen retrieval (sodium citrate). Incubation: 1 hour at RT; detection DAB.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

IHC-P: Human placenta

属性

形式	Liquid
纯化	Purified from ascites by protein-A affinity chromatography.
纯度	> 95% (by SDS-PAGE)
缓冲液	PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂	15 mM Sodium azide
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3133 Human HLA-E Swiss-port # P13747 Human HLA class I histocompatibility antigen, alpha chain E
基因名称	HLA-E
全名	major histocompatibility complex, class I, E
背景介绍	HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
生物功能	Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules. [UniProt]
研究领域	Immune System antibody
预测分子量	40 kDa

检测图片 (1)

ARG63017 anti-HLA E antibody [MEM-E/02] IHC-P image
Immunohistochemistry: Colorectal epithelial cells between the four groups: normal mucosa (Negative expression), adenoma (Negative expression), early cancer group (weak positive) and advanced cancer group (strong positive) stained with ARG63017 anti-HLA E antibody [MEM-E/02].
From Renxiang Huang et al. Oncol Lett (2017), doi: 10.3892/ol.2017.5891, Fig. 5.

参考文献

Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells

IHC-P, WB / Human

Renxiang Huang et al.
Oncol Lett., (2017)