anti-Hsp 40 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Hsp 40
  • 反应物种: Hu
  • 应用: ICC/IF, IP, WB
  • 特异性: Detects ~40kDa.
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 靶点名称: Hsp 40
  • 抗原物种: Human
  • 抗原: Recombinant purified Human HSP40
  • Brand:
CAT.NO. : ARG22196
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Hsp 40
反应物种Hu
应用ICC/IF, IP, WB
特异性Detects ~40kDa.
宿主Rabbit
克隆Polyclonal
靶点名称Hsp 40
抗原物种Human
抗原Recombinant purified Human HSP40
偶联标记Un-conjugated
別名Hsp40; HSP40; DnaJ protein homolog 1; hDj-1; HSPF1; Human DnaJ protein 1; Hdj1; RSPH16B; Heat shock 40 kDa protein 1; DnaJ homolog subfamily B member 1; Heat shock protein 40; Sis1
应用说明
应用建议
应用推荐稀释比
ICC/IF1:100
IPAssay-dependent
WB1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Rabbit antiserum.
缓冲液Rabbit Antiserum
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3337 Human DNAJB1

Swiss-port # P25685 Human DnaJ homolog subfamily B member 1

基因名称DNAJB1
全名DnaJ (Hsp40) homolog, subfamily B, member 1
背景介绍This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
生物功能Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP. [UniProt]
细胞定位Cytoplasm, Nucleus
预测分子量38 kDa
检测图片 (3)
  • ARG22196 anti-Hsp 40 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22196 anti-Hsp 40 antibody WB image

    Western blot: Human HeLa cell lysates stained with ARG22196 anti-Hsp 40 antibody at 1:1000 dilution.

  • ARG22196 anti-Hsp 40 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22196 anti-Hsp 40 antibody (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

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