anti-Hsp 47 antibody [1C4-1A6]

CAT.NO. : ARG22207

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Size:

100 μg

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Product Details

概述

产品描述	Mouse Monoclonal antibody [1C4-1A6] recognizes Hsp 47
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1C4-1A6
同位型	IgG1, kappa
靶点名称	Hsp 47
抗原物种	Human
抗原	Full length Human HSP47
偶联标记	Un-conjugated
別名	SERPINH1; Serpin Family H Member 1; HSP47; SERPINH2; CBP1; CBP2; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Cell Proliferation-Inducing Gene 14 Protein; Rheumatoid Arthritis-Related Antigen RA-A47; Arsenic-Transactivated Protein 3; Collagen Binding Protein 1; 47 KDa Heat Shock Protein; Heat Shock Protein 47; Serpin H1; Colligin; AsTP3; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2); Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2; Rheumatoid Arthritis Antigen A-47; Collagen-Binding Protein; Colligin-1; Colligin-2; RA-A47; PIG14; PPROM; OI10; Gp46

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:100
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂	0.09% Sodium azide
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 871 Human SERPINH1 Swiss-port # P50454 Human Serpin H1
基因名称	SERPINH1
全名	Serpin Family H Member 1
背景介绍	This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011]
生物功能	Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. [UniProt]
细胞定位	Endoplasmic reticulum. [UniProt]
预测分子量	46 kDa
翻译后修饰	Acetylationl; Glycoprotein Phosphoprotein. [UniProt]

检测图片 (3)

ARG22207 anti-Hsp 47 antibody [1C4-1A6] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
ARG22207 anti-Hsp 47 antibody [1C4-1A6] WB image
Western blot: 1) MW ladder, 20 µg of A431 lysates stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (TBS for 60 min at RT) at 2) 1:250, 3) 1:500, or 4) 1:1000 dilutions. Block: 5% milk + TBST 1hr at RT.
ARG22207 anti-Hsp 47 antibody [1C4-1A6] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

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