anti-Hsp 60 antibody [LK1]
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Key features and details
- 产品描述:
- 反应物种:
- 不反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [LK1] recognizes Hsp 60 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 不反应物种 | Bacteria |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | LK1 |
| 同位型 | IgG1, kappa |
| 靶点名称 | Hsp 60 |
| 抗原物种 | Human |
| 抗原 | Recombinant Human Hsp60 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Heat shock protein 60; HuCHA60; 60 kDa heat shock protein, mitochondrial; GROEL; P60 lymphocyte protein; 60 kDa chaperonin; HSP-60; Mitochondrial matrix protein P1; HLD4; SPG13; HSP60; Chaperonin 60; Hsp60; CPN60; HSP65 |
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 0.1 mg/ml BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | HSPD1 |
| 全名 | heat shock 60kDa protein 1 (chaperonin) |
| 背景介绍 | This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010] |
| 生物功能 | Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. [UniProt] |
| 细胞定位 | Mitochondria in cytoplasm |
| 研究领域 | Controls and Markers antibody; Signaling Transduction antibody; Mitochondrial Marker antibody |
| 预测分子量 | 61 kDa |
ARG55956 anti-Hsp 60 antibody [LK1] WB image
Western blot: 20 µg of 293T, Mouse brain and Rat brain lysates stained with ARG55956 anti-Hsp 60 antibody [LK1] at 0.4 µg/ml dilution.
ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded Human breast carcinoma stained with ARG55956 anti-Hsp 60 antibody [LK1].
ARG55956 anti-Hsp 60 antibody [LK1] ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG55956 anti-Hsp 60 antibody [LK1] at 1:200 dilution. Left: primary antibody (green). Middle: DAPI (blue). Right: Merge.
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
ARG55956 anti-Hsp 60 antibody [LK1] IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin-embedded pancreas tissue stained with ARG55956 anti-Hsp 60 antibody [LK1].
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