anti-Hsp 70 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Hsp 70
  • 反应物种: Hu, Ms, Rat, Bov, Carp, Dog, Fsh, Gpig, Hm, Mk, Pig, Plnt, Shark, Sheep
  • 应用: ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
  • 特异性: Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 靶点名称: Hsp 70
  • 抗原物种: Human
  • 抗原: Full length HSP70 protein
  • Brand:
CAT.NO. : ARG22291
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Hsp 70
反应物种Hu, Ms, Rat, Bov, Carp, Dog, Fsh, Gpig, Hm, Mk, Pig, Plnt, Shark, Sheep
应用ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性Detects a ~70kDa. May cross-react with HSC70 at lower dilutions.
宿主Rabbit
克隆Polyclonal
靶点名称Hsp 70
抗原物种Human
抗原Full length HSP70 protein
偶联标记Un-conjugated
別名Heat shock 70 kDa protein 1A; HSPA1; HSP70I; Heat shock 70 kDa protein 1; HSP70-1A; HEL-S-103; HSP70.1; HSP72; HSP70-1
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
ICC/IF1:100
IHC-Fr1:100
IHC-P1:100
IP1:100
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
抗菌剂0.09% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 193740 Mouse HSPA1A

GeneID: 281825 Bovine HSPA1A

GeneID: 282254 Bovine HSPA1A

基因名称HSPA1A
全名heat shock 70kDa protein 1A
背景介绍This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]
生物功能In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. [UniProt]
细胞定位Cytoplasm
预测分子量70 kDa
翻译后修饰In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.
检测图片 (7)
  • ARG22291 anti-Hsp 70 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22291 anti-Hsp 70 antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22291 anti-Hsp 70 antibody IHC image

    Immunohistochemistry: Formalin fixed Mouse Inflamed colon stained with ARG22291 anti-Hsp 70 antibody at 1:1000 dilution (12 hours). Counterstain: Methyl Green at 200uL for 2 min at RT.

  • ARG22291 anti-Hsp 70 antibody WB image

    Western blot: Mouse Pam212 cells stained with ARG22291 anti-Hsp 70 antibody at 1:1000 dilution.

  • ARG22291 anti-Hsp 70 antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22291 anti-Hsp 70 antibody (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22291 anti-Hsp 70 antibody IHC image

    Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22291 anti-Hsp 70 antibody (green) at 1:100 dilution (1 hour).

  • ARG22291 anti-Hsp 70 antibody IHC image

    Immunohistochemistry: Formalin fixed Human colon carcinoma stained with ARG22291 anti-Hsp 70 antibody at 1:50000 dilution (12 hours). Counterstain: Methyl Green at 200uL for 2 min at RT.

  • ARG22291 anti-Hsp 70 antibody WB image

    Western blot: Human, Rat brain cell lysates stained with ARG22291 anti-Hsp 70 antibody at 1:10000 dilution.

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