Anti-Human Ig Kappa Light Chain Rabbit Monoclonal Antibody [Clone RM126]; 31-1028-00

Key features and details

  • Uniprot: P01834
  • Gene ID: 3514
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1028-00
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Product Details
Product Details
Product NameAnti-Human Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM126
Product DescriptionRabbit monoclonal to Human Kappa Light Chain
Catalog No.31-1028-00
Clone NameRM126
Product Data SheetAnti-Human Kappa Light Chain Rabbit Monoclonal Antibody Data Sheet RM126
SpecificityRM126reacts to the kappa light chain of human immunoglobulins, and does not react to the lambda light chain. RM126 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG.
ImmunogenHuman IgG
ApplicationImmunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species ReactivityHuman
ConjugateNone
Intended UseResearch Use Only
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Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size100 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 0.05 µg/mL – 0.2 µg/mL;
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL;
Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL.
Keywords
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Product Images

Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig kappa light chain rabbit monoclonal antibody, Clone RM126.

ELISA

ELISA showing RM126 reacts only to the kappa light chain of human immunoglobulins, and not to lambda light chain, mouse IgG, rat IgG, or goat IgG.

A

A titer ELISA using RM126. The plate was coated with different amounts of human IgG4κ. A serial dilution of RM126 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA

ELISA showing RM126 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM126 and a monkey IgG binding antibody (positive control) was used as the detection antibody.

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