Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [Clone RM129], 32-1031-00
![Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [Clone RM129], 32-1031-00](/upload/image/products/Fig2_HuLC_e.png)
![Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [Clone RM129], 32-1031-00](/upload/image/products/Fig3a_HuLC_e.png)
![Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [Clone RM129], 32-1031-00](/upload/image/products/Fig3b_HuLC_e.png)
![Anti-Human Ig Light Chain Rabbit Monoclonal Antibody [Clone RM129], 32-1031-00](/upload/image/products/Fig1_HuLC_e.png)
Key features and details
- Uniprot:
- Gene ID:
- Host Species:
- Species Reactivity:
- Concentration:
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Brand:
Product Details
| Product Name | Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129 |
| Product Description | Rabbit monoclonal to Human Immunoglobulin Light Chains |
| Catalog No. | 32-1031-00 |
| Clone Name | RM129 |
| Product Data Sheet | Anti-Human Ig Light Chain Rabbit Monoclonal Antibody Data Sheet RM129 |
| Specificity | RM129reacts to both kappa and lambda light chain of human immunoglobulins. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG |
| Application | ELISA Immunohistochemistry (IHC) Immunocytochemistry (ICC) Flow Cytometry (FC) |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Keywords | |
| Usage | ELISA: 0.1 µg/mL - 0.5 µg/mL; Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL.; Flow Cytometry (FC): 0.5 µg/mL - 2 µg/mL. |
| . | |
 | ELISA shows that clone RM129 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM129 and a monkey IgG reacting antibody (positive control) was used as the detection antibodies. |
 | ELISA shows that clone RM129 reacts only to the kappa and lambda light chains in all human immunoglobulins, and does not react to mouse IgG, rat IgG, or goat IgG. |
 | A titer ELISA using RM129. The plate was coated with different amounts of human IgG4k. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM129. The plate was coated with different amounts of human IgG3λ. A serial dilution of RM129 was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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