Anti-Human IgG Rabbit Monoclonal Antibody [Clone RM116]; 31-1018-00
![Anti-Human IgG Rabbit Monoclonal Antibody [Clone RM116]; 31-1018-00](/upload/image/products/RM116_image3-e1453506688258.jpg)
![Anti-Human IgG Rabbit Monoclonal Antibody [Clone RM116]; 31-1018-00](/upload/image/products/RM116_image2_ELISA.png)
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Key features and details
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Product Details
| Product Name | Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody, Clone RM116 |
| Product Description | Rabbit Monoclonal to Human Gamma Heavy Chain |
| Catalog No. | 31-1018-00 |
| Clone Name | RM116 |
| Product Data Sheet | Anti-Human Gamma Heavy Chain Rabbit Monoclonal Antibody Data Sheet RM116 |
| Specificity | RM116 reacts to the Fc region of all gamma heavy chains of human immunoglobulins, including γ1, γ2, γ3, and γ4. No cross reactivity with human IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG |
| Application | ELISA Immunocytochemistry (ICC) Immunohistochemistry (IHC) |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. |
| Keywords | |
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 | Immunohistochemistry of Human Tonsil using Anti-Gamma Heavy Chain antibody, Clone RM116. |
 | ELISA of human immunoglobulins shows RM116 reacted to the γ1, γ2, γ3, γ4 heavy chain of human IgGs, and the Fc of human IgG. No cross reactivity with other human heavy chains, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM116. The plate was coated with different amounts of human IgG1. A serial dilution of RM116 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | Sandwich ELISA using RM116 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody (1 µg/mL), followed by an alkaline phosphatase conjugated streptavidin. |
 | Sandwich ELISA using RM116 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody (1 µg/mL), followed by an alkaline phosphatase conjugated streptavidin. |
 | Cross-activity data of RevMAb anti-human IgG antibodies against monkey IgG. ELISA plate was coated with 0.1 µg/well (2 µg/mL, 50 µL/well) of Rhesus monkey IgG (protein A purified from serum).A serial dilution of anti-human IgG (RM116), anti-human IgG1 (RM117), anti-human IgG2 (RM118), anti-human IgG3 RM119), anti-human IgG4 (RM120), anti-human kappa (RM126), and anti-human lambda (RM127) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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