anti-IKB alpha phospho (Ser32 / Ser36) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes IKB alpha phospho (Ser32 / Ser36) |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IKB alpha |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IκB-α. |
| 偶联标记 | Un-conjugated |
| 別名 | NFKBI; I-kappa-B-alpha; IkB-alpha; NF-kappa-B inhibitor alpha; Major histocompatibility complex enhancer-binding protein MAD3; IKBA; IkappaBalpha; MAD-3 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
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| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | NFKBIA |
| 全名 | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| 背景介绍 | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription. |
| 生物功能 | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30037 Phospho IKB alpha Antibody Duo (Total, pS32/36) ARG30205 NFkB Activation Antibody Panel Related products: IKB alpha antibodies; IKB alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Treatment of Obesity with Celastrol Tumor microenvironments are shown to affect progression of several cancer subtypes Immune signaling protein TLR4 has opposing roles in breast cancer development |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody; NF-kB Activation Study antibody |
| 预测分子量 | 36 kDa |
| 翻译后修饰 | Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation. Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability. Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36. Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. |
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image
Western blot: Rat cardiac stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody atdilution.
From Yongjun Mao et al. Biomed Pharmacother. (2020), doi: 10.1016/j.biopha.2019.109280, Fig. 6D.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody WB image
Western blot: Extracts from HeLa cells untreated or treated with TNF stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast carcinoma tissue stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody (left) or the same antibody preincubated with blocking peptide (right).
ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with ARG51651 anti-IKB alpha phospho (Ser32 / Ser36) antibody.
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