anti-IKK alpha antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原:
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Brand:
CAT.NO. : ARG54650
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes IKK alpha |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, ICC/IF, WB |
| 特异性 | Antibody has no cross response to IKKb or IKKg. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | IKK alpha |
| 抗原 | Synthetic peptide (19 aa) within the last 50 aa of Human IKK alpha. |
| 偶联标记 | Un-conjugated |
| 別名 | Conserved helix-loop-helix ubiquitous kinase; IKK-A; TCF16; IKK1; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase 1; IkappaB kinase; Transcription factor 16; EC 2.7.11.10; Nuclear factor NF-kappa-B inhibitor kinase alpha; IkBKA; IKBKA; IKK-alpha; NFKBIKA; IKKA; TCF-16; I-kappa-B kinase alpha |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | HeLa Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # O15111 Human Inhibitor of nuclear factor kappa-B kinase subunit alpha |
|---|---|
| 基因名称 | CHUK |
| 全名 | conserved helix-loop-helix ubiquitous kinase |
| 背景介绍 | IKK alpha Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα and IKKβ). IKKα and IKKβ interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IkB-alpha. IKKα is expressed in variety of human tissues. | |
| 产品亮点 | Related Antibody Duos and Panels: ARG30255 IKB alpha degradation Antibody Panel (IKK alpha, IKK beta, IKB alpha) Related products: IKK alpha antibodies; IKK alpha Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related poster download: Toll-like Receptor.pdf |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody; IκB alpha degradation Study antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated. (Microbial infection) Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. |
检测图片 (3)
ARG54650 anti-IKK alpha antibody WB image
Western blot: 30 μg of HeLa cell lysates 1) untreated or 2) treated with 5 ng/ml TNF-a for 5 min, stained with ARG54650 anti-IKK alpha antibody at 1:500 dilution.
ARG54650 anti-IKK alpha antibody ICC/IF image
Immunocytochemistry: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 1μg/ml.
ARG54650 anti-IKK alpha antibody ICC/IF image
Immunofluorescence: Jurkat cells stained with ARG54650 anti-IKK alpha antibody at 10 μg/ml.
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