anti-IL1 alpha antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes IL1 alpha
  • 反应物种: Hu, Rat
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IL1 alpha
  • 抗原物种: Human
  • 抗原: E.coli derived Recombinant Human IL-1α.(SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA)
  • Brand:
CAT.NO. : ARG56666
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes IL1 alpha
反应物种Hu, Rat
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称IL1 alpha
抗原物种Human
抗原E.coli derived Recombinant Human IL-1α.
(SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA)
偶联标记Un-conjugated
別名IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56776 as a detection antibody
IHC-P1.0 µg/ml
Neut1.0 - 2.0 µg/ml (To yield [ND50] of the biological activity of hIL - 1α (20 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 24493 Rat IL1A

GeneID: 3552 Human IL1A

Swiss-port # P01583 Human Interleukin-1 alpha

Swiss-port # P16598 Rat Interleukin-1 alpha

基因名称IL1A
全名interleukin 1, alpha
背景介绍The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
生物功能Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
产品亮点Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
预测分子量31 kDa
检测图片 (6)
  • ARG56666 anti-IL1 alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56666 anti-IL1 alpha antibody concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.

  • ARG56666 anti-IL1 alpha antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-1α stained with ARG56666 anti-IL1 alpha antibody, under reducing conditions.

  • ARG56666 anti-IL1 alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56666 anti-IL1 alpha antibody concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.

  • ARG56666 anti-IL1 alpha antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of normal Human skin. The recommended ARG56666 anti-IL1 alpha antibody concentration is 1.0 µg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.

  • ARG56666 anti-IL1 alpha antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-1α stained with ARG56666 anti-IL1 alpha antibody, under non-reducing conditions.

  • ARG56666 anti-IL1 alpha antibody standard curve image

    Sandwich ELISA: ARG56666 anti-IL1 alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56776 anti-IL1 alpha antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

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