anti-IL2 antibody [1H10-1-1]

CAT.NO. : ARG56599

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Size:

200 μg 100 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [1H10-1-1] recognizes IL2
反应物种	Hu
应用	ELISA, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	1H10-1-1
同位型	IgG1, kappa
靶点名称	IL2
抗原物种	Human
抗原	E.coli derived Recombinant Human IL-2. (MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT)
偶联标记	Un-conjugated
別名	TCGF; IL-2; lymphokine; Interleukin-2; Aldesleukin; T-cell growth factor

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 4.0 - 8.0 µg/ml as a capture antibody
IHC-P	2.5 µg/ml - 5.0 µg/ml
WB	1.0 - 2.0 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3558 Human IL2 Swiss-port # P60568 Human Interleukin-2
基因名称	IL2
全名	interleukin 2
背景介绍	The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
生物功能	Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProt]
预测分子量	18 kDa

检测图片 (4)

ARG56599 anti-IL2 antibody [1H10-1-1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56599 anti-IL2 antibody [1H10-1-1] concentration is 2.5 µg/ml-5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56599 anti-IL2 antibody [1H10-1-1] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-2 stained with ARG56599 anti-IL2 antibody [1H10-1-1], under reducing conditions.
ARG56599 anti-IL2 antibody [1H10-1-1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56599 anti-IL2 antibody [1H10-1-1] concentration is 2.5 µg/ml-5.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
ARG56599 anti-IL2 antibody [1H10-1-1] standard curve image
Sandwich ELISA: ARG56599 anti-IL2 antibody [1H10-1-1] as a capture antibody at 4.0 - 8.0 µg/ml combined with anti-IL2 antibody (Biotin) as a detection antibody at ~ 1.0 - 2.0 µg/ml. Results of a typical standard run with optical density.

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