anti-IL3 antibody [5A4-1-16]

CAT.NO. : ARG65985

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Size:

100 μg

Product Details

概述

产品描述	Mouse Monoclonal antibody [5A4-1-16] recognizes IL3
反应物种	Hu
应用	ELISA, Neut, WB
宿主	Mouse
克隆	Monoclonal
克隆号	5A4-1-16
同位型	IgG
靶点名称	IL3
抗原物种	Human
抗原	E. coli derived recombinant Human IL3. (APMTQTTSLK TSWVNCSNMI DEIITHLKQP PLPLLDFNNL NGEDQDILME NNLRRPNLEA FNRAVKSLQN ASAIESILKN LLPCLPLATA APTRHPIHIK DGDWNEFRRK LTFYLKTLEN AQAQQTTLSL AIF)
偶联标记	Un-conjugated
別名	MCGF; Mast cell growth factor; P-cell-stimulating factor; Hematopoietic growth factor; IL-3; Interleukin-3; Multipotential colony-stimulating factor; MULTI-CSF

应用说明

应用建议

应用	推荐稀释比
ELISA	Sandwich: 4.0 - 8.0 µg/ml with ARG65999 as a detection antibody
Neut	0.007 - 0.010 µg/ml (To yield [ND50] of the biological activity of Human IL-3 (1.0 ng/ml) )
WB	0.25 - 0.50 µg/ml

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.2)
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3562 Human IL3 Swiss-port # P08700 Human Interleukin-3
基因名称	IL3
全名	interleukin 3
背景介绍	The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
生物功能	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes. [UniProt]
预测分子量	17 kDa

检测图片 (3)

ARG65985 anti-IL3 antibody [5A4-1-16] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-3 stained with ARG65985 anti-IL3 antibody [5A4-1-16], under non-reducing conditions.
ARG65985 anti-IL3 antibody [5A4-1-16] standard curve image
Sandwich ELISA: ARG65985 anti-IL3 antibody [5A4-1-16] as a capture antibody at 4.0 - 8.0 µg/ml combined with ARG65999 anti-IL3 antibody (Biotin) as a detection antibody at ~ 0.50 - 1.0 µg/ml. Results of a typical standard run with optical density reading at 405 - 650 nm.
ARG65985 anti-IL3 antibody [5A4-1-16] WB image
Western blot: 250 - 0.24 ng (left to right) of Human IL-3 stained with ARG65985 anti-IL3 antibody [5A4-1-16], under reducing conditions.

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