anti-IL33 antibody (Biotin)

Key features and details

  • 产品描述: Biotin-conjugated Rabbit Polyclonal antibody recognizes IL33
  • 反应物种: Hu
  • 应用: ELISA, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IL33
  • 抗原物种: Human
  • 抗原: E. coli derived recombinant Human IL33.(SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET)
  • Brand:
CAT.NO. : ARG66127
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Product Details
概述
产品描述Biotin-conjugated Rabbit Polyclonal antibody recognizes IL33
反应物种Hu
应用ELISA, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称IL33
抗原物种Human
抗原E. coli derived recombinant Human IL33.
(SITGISPITE YLASLSTYND QSITFALEDE SYEIYVEDLK KDEKKDKVLL SYYESQHPSN ESGDGVDGKM LMVTLSPTKD FWLHANNKEH SVELHKCEKP LPDQAFFVLH NMHSNCVSFE CKTDPGVFIG VKDNHLALIK VDSSENLCTE NILFKLSET)
偶联标记Biotin
別名95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270
应用说明
应用建议
应用推荐稀释比
ELISADirect: 0.25 - 1.0 µg/ml
Sandwich: 0.25 - 1.0 µg/ml with ARG66126 as a capture antibody
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified by affinity chromatography.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 90865 Human IL33

Swiss-port # O95760 Human Interleukin-33

基因名称IL33
全名interleukin 33
背景介绍The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
生物功能Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.

In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProt]
预测分子量31 kDa
翻译后修饰The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
检测图片 (4)
  • ARG66127 anti-IL33 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human IL-33 stained with ARG66127 anti-IL33 antibody (Biotin), under non-reducing conditions.

  • ARG66127 anti-IL33 antibody (Biotin) standard curve image

    Direct ELISA: ARG66127 anti-IL33 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66127 anti-IL33 antibody (Biotin) WB image

    Western blot: 250 - 0.24 ng of Human IL-33 stained with ARG66127 anti-IL33 antibody (Biotin), under reducing conditions.

  • ARG66127 anti-IL33 antibody (Biotin) standard curve image

    Sandwich ELISA: ARG66127 anti-IL33 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66126 anti-IL33 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

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