anti-IL4 antibody [SQab18131]

CAT.NO. : ARG66429

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Size:

100 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [SQab18131] recognizes IL4
反应物种	Hu
应用	ELISA, FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	SQab18131
同位型	IgG1, kappa
靶点名称	IL4
抗原物种	Human
抗原	Recombinant Human IL4 protein.
偶联标记	Un-conjugated
別名	BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂	0.01% Thimerosal
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 3565 Human IL4 Swiss-port # P05112 Human Interleukin-4
基因名称	IL4
全名	interleukin 4
背景介绍	The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt]
细胞定位	Secreted. [UniProt]
预测分子量	17 kDa

检测图片 (4)

ARG66429 anti-IL4 antibody [SQab18131] ICC/IF image
Immunofluorescence: MCF-7 cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66429 anti-IL4 antibody [SQab18131] (green) at 1:400 and cell nuclei were stained with Hoechst 33342 (blue).
ARG66429 anti-IL4 antibody [SQab18131] FACS image
Flow Cytometry: MCF-7 cells were stained with ARG66429 anti-IL4 antibody [SQab18131] at 1 µg/ml (red) and without antibody control (black).
ARG66429 anti-IL4 antibody [SQab18131] WB image
Western blot: 100 ng of Recombinant IL4 protein and 60 µg of 293T cell lysate stained with ARG66429 anti-IL4 antibody [SQab18131] at 1:2000 dilution.
ARG66429 anti-IL4 antibody [SQab18131] ELISA image
ELISA: Titration curve of ARG66429 anti-IL4 antibody [SQab18131]. Red: IL4; Black: BSA (negative control).

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