anti-IL5 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes IL5
  • 反应物种: Hu
  • 应用: ELISA, IHC-P, Neut, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: IL5
  • 抗原物种: Human
  • 抗原: E. coli derived recombinant Human IL5.(MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES)
  • Brand:
CAT.NO. : ARG66000
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes IL5
反应物种Hu
应用ELISA, IHC-P, Neut, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称IL5
抗原物种Human
抗原E. coli derived recombinant Human IL5.
(MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES)
偶联标记Un-conjugated
別名Eosinophil differentiation factor; EDF; IL-5; TRF; T-cell replacing factor; B-cell differentiation factor I; Interleukin-5
应用说明
应用建议
应用推荐稀释比
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66001 as a detection antibody
IHC-P0.25 µg/ml
Neut0.05 - 0.15 µg/ml (To yield [ND50] of the biological activity of Human IL-5 (5.0 ng/ml) )
WB0.1 - 0.2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.2)
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 3567 Human IL5

Swiss-port # P05113 Human Interleukin-5

基因名称IL5
全名interleukin 5
背景介绍This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]
生物功能Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells. [UniProt]
预测分子量15 kDa
检测图片 (4)
  • ARG66000 anti-IL5 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human colon and rectum adenocarcinoma. The recommended ARG66000 anti-IL5 antibody concentration is 0.25 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • ARG66000 anti-IL5 antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-5 stained with ARG66000 anti-IL5 antibody, under non-reducing conditions.

  • ARG66000 anti-IL5 antibody standard curve image

    Sandwich ELISA: ARG66000 anti-IL5 antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66001 anti-IL5 antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.

  • ARG66000 anti-IL5 antibody WB image

    Western blot: 250 - 0.24 ng of Human IL-5 stained with ARG66000 anti-IL5 antibody, under reducing conditions.

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